Comparison of Estrogen β Receptor Expression and the Addition of Various Dose of Geistein to HUVEC Endothel Cell which Exposed to Oxidative Stress

Abstract

Objective: To compare β estrogen receptor on HUVEC endothelial for each Genistein dose addition with β-estradiol physiologic dose. Methode: Experimental study performed in Physiology Laboratorium, Medical Faculty of Brawijaya University Malang, HUVEC culture exposed by glucose as a dysfunction endothelial model. Endothel collected from female newborn umbilicus was cultured to make a confluent culture. The culture was divided into 7 groups: 1. HUVEC without treatment, 2. HUVEC + glucose 33 mM, 3. HUVEC + glucose 33 mM + 17β estradiol , 4. HUVEC + glucose 33 mM+ Genistein 2.5 μm. 5. HUVEC + Glucose 33 mM + Genistein 5 μm, 6. HUVEC + Glucose 33 mM + Genistein 7,5 μm, 7. HUVEC + Glucose 33 mM + Genistein 10 μm. Culture then was incubated in 37°C and stained with immunohistochemistry, using primary antibody of β estrogen receptor. The expression was observed on the -15, 30, 60, 120 and 240th minute. The datas from observation result was analysed with ANOVA test and correlation test. Result: Estrogen receptor comparison between group 1 was significantly different with group 2, 3, 6, 7 and not significantly different with groups 4, 5. Estrogen receptor between group 2 was significantly different with group 4, 5 but not significantly different with groups 3, 6, 7. Estrogen receptor comparison between group 3 was significantly different with group 4, 5 and not significantly different with groups 6, 7. Estrogen receptor comparison between group 4 was significantly different with group 6, 7 and not significantly different with groups 5. Estrogen receptor comparison between group 5 was significantly different with group 6, 7. Estrogen receptor comparison between group 6 was not significantly different with group 7. Isoflavon Genistein dose increase on 30, 60 dan 120 minutes will reduce β estrogen receptor expression on endothelial HUVEC and on 15 and 240 minutes shows that there was no significant correlation with β estrogen receptor expression on endothel HUVEC. Conclusion: There was significant expression distinction of ERβ in glucose induced HUVEC exposed by 17β estradiol compared with those exposed by Genistein but not in Genistein dose 7.5 and 10 μm. The lower of Isoflavon Genistein dose in the -30, 60 dan 120 minute will cause expression of β estrogen receptor in HUVEC endothelial cell that tends to be higher. [Indones J Obstet Gynecol 2010; 34-1:24-30] Keywords: β estrogen receptor, HUVEC, genistein, estradiol.