Impact of Freeze-Dried Amnion Membrane and Human Amnion Stem Cell Seeding on TGF-B and Collagen Type III in Vesicovaginal Fistula

Pengaruh Pemberian Membran Amnion Kering Beku dan Seeding Sel Punca Amnion terhadap TGF B dan Kolagen Tipe III pada Penjahitan Fistula Vesikovaginalis

  • Jojor Sihotang Department Obstetry and Gynecology, Faculty of Medicine, Riau University
  • Eighty Mardiyan Faculty of Medicine, Airlangga univeristy/ Dr. Soetomo General Hospital Surabaya
  • Eighty Mardiyan Faculty of Medicine, Airlangga univeristy/ Dr. Soetomo General Hospital Surabaya
  • Widjiati Widjiati Faculty of Veterinary, Airlangga univeristy
  • Amiruddin Hidayatullah Faculty of Medicine, Riau University

Abstract

Objective: to analize how freeze-dried amniotic membrane and human amniotic stem cell seeding affect TGF B and type III collagen expression in suturing a New Zealand rabbit vesicovaginal fistula model.

Metode: This experimental study employed New Zealand rabbits and a vesicovaginal fistula model with a post-test only control group design. The rabbits were divided into 3 groups: vesicovaginal fistula suturing alone, suturing with freeze-dried amniotic membrane, and suturing with freeze-dried amnion-seeded stem cells. After 7 days of treatment, specimens near the repaired vesicovaginal fistula were collected for immunohistochemical analysis of TGF B and collagen type III expression.

Result: TGF B expression was significantly higher in the freeze-dried amniotic membrane with stem cell seeding group (p=0.001) compared to the freeze-dried amniotic membrane without stem cell seeding group (p=0.017) and the suturing-only group (p=0.049). Additionally, type III collagen expression was significantly elevated in the freeze-dried amnion membrane and stem cell seeding group (p=0.001) compared to the freeze-dried amnion group without stem cell seeding (p=0.09) and the suturing-only group (p=0.026).

Conclusion: The expression of TGF B and type III collagen was higher in rabbits with vesicovaginal fistulas treated using freeze-dried amnion and amniotic stem cell seeding compared to those without amniotic stem cell seeding and vesicovaginal fistula suturing alone.

Keywords: Vesicovaginal Fistula, Freeze-dried Amnion, Stem cell

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Author Biographies

Eighty Mardiyan, Faculty of Medicine, Airlangga univeristy/ Dr. Soetomo General Hospital Surabaya

Departement of Obstetry and Gynecology

Eighty Mardiyan, Faculty of Medicine, Airlangga univeristy/ Dr. Soetomo General Hospital Surabaya

Departement of Obstetry and Gynecology

Widjiati Widjiati, Faculty of Veterinary, Airlangga univeristy

Departement of Anatomy

Amiruddin Hidayatullah, Faculty of Medicine, Riau University

Departement of Obstetry and Gynecology

Published
2024-10-30
Section
Research Article