RELA mRNA Expression in Epithelial Ovarian Cancer (EOC): Correlation with rs11820062 Gene Variant

  • Benedikta Diah Saraswati Universitas Indonesia
  • Dwi Anita Suryandari Universitas Indonesia
  • Ria Kodariah Universitas Indonesia
  • Dewi SUKMAWATI Universitas Indonesia
  • Luluk Yunaini
  • Primariadewi Rustamadji

Abstract

Objectivity: To examine the distribution of RELA rs11820062 and its correlation to RELA mRNA expression in low-grade and high-grade epithelial ovarian cancer (EOC)’s patients from Rumah Sakit Cipto Mangunkusumo, Indonesia.

Materials and Methods: This study is cross-sectional with a total of 65 healthy subjects and 80 ovarian biopsies (15 ovarian cysts as expression calibrators, 29 low-grade EOC, and 36 high-grade EOC) were used in this study. The distribution of genotypes and alleles was analyzed using ARMS PCR. The mRNA expressions of RELA were determined by real-time polymerase chain reaction (qPCR) analysis.

Results: There was no significant difference between genotype and allele distributions for RELA rs11820062 in normal and case group. RELA relative mRNA expression was significantly higher in low-grade and high-grade EOC compared to in ovarian cysts (p<0.01). RELA rs11820062 CC genotype correlated to higher RELA mRNA relative expression and the TT genotype of RELA rs11820062 correlated with lower RELA mRNA relative expression in low-grade and high-grade EOC.

Conclusion: RELA overexpression in EOC may be due to the C allele in rs11820062, which individuals with CC genotype correlated with higher RELA expression in low-grade and high-grade EOC. In contrast, individuals with the T allele of RELA rs11820062 had a protective effect against EOC risk because the RELA TT genotype tended to have a lower RELA mRNA expression in EOC.

 

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Published
2023-04-30
Section
Research Article