Serum Nephrin Levels in Severe Preeclampsia: A Cross-Sectional Study

Kadar Nephrin Serum pada Preeklamsia Berat: Sebuah Studi Potong Lintang

  • Meice Fitrina RS. Hasan Sadikin
  • Sofie R. Krisnadi Faculty of Medicine Universitas Padjadjaran Dr. Hasan Sadikin General Hospital Bandung
  • Hartanto Bayuaji Faculty of Medicine Universitas Padjadjaran Dr. Hasan Sadikin General Hospital Bandung

Abstract

Abstract

Objective: To determine differences in serum nephrin levels in severe preeclampsia compared to normal pregnancy and also its correlation with systolic and diastolic blood pressure and proteinuria.
Methods: This study is an analytical observational with cross sectional study. The observation group consisted of severe preeclampsia (n= 30) and normal pregnancy group as a control (n= 30). Both groups measured systolic and diastolic blood pressure, proteinuria and serum nephrin using the Human NPHN (Nephrin) ELISA Kit. Statistical test were performed with Mann-Whitney test and the Spearman’s rank test. A value of p<0.05 was considered significant. The study was conducted in the Obstetric Clinic Inward and Laboratorium Department of Clinical Pathology Dr. Hasan Sadikin General Hospital/Faculty of Medicine Universitas Padjadjaran on March–May 2019.
Results: Levels of serum nephrin in the severe preeclampsia group were significantly higher than in normal pregnancies (6.4 ng/mL vs 4.2 ng/mL; p= 0.014). There is a positive weak correlation but statistically significant between serum nephrin with systolic blood pressure (r= 0.36; p= 0.02) but not significant to diastolic blood pressure (r= 0.3; p= 0.05). There is no significant correlation was found between serum nephrin levels and proteinuria (r= 0.18; p= 0.54).
Conlusions:  Levels of serum nephrin in the severe preeclampsia group were significantly higher than in normal pregnancies and there is a correlation between serum nephrin with systolic blood pressure.

Keywords: blood pressure, proteinuria, serum nephrin, podocyte, severe preeclampsia.

Abstrak

Tujuan: Untuk mengetahui perbedaan kadar nephrin serum pada preeklamsia berat dibandingkan dengan kehamilan normal dan juga hubungannya dengan tekanan darah sistolik dan diastolik serta proteinuria.
Metode: Penelitian ini bersifat analitik observasional dengan pendekatan potong silang. Kelompok pengamatan terdiri dari kelompok preeklamsia berat (n= 30) dan kelompok kehamilan normal sebagai kontrol (n= 30). Pada kedua kelompok dilakukan pengukuran tekanan darah sistolik dan diastolik, proteinuria serta pengukuran kadar nephrin serum menggunakan Human NPHN (Nephrin) ELISA Kit. Uji statistik dilakukan dengan uji Mann-Whitney dan uji rank Spearman. Nilai p<0,05 dianggap bermakna. Penelitian dilakukan di ruang perawatan obstetri FKUP/RSHS dan Laboratorium Patologi Klinik FKUP/RSHS pada bulan Maret-Mei 2019.
Hasil: Rerata kadar nephrin serum pada kelompok preeklamsia berat lebih tinggi secara bermakna dibandingkan kehamilan normal (6,4 ng/mL vs 4,2 ng/mL; p= 0,014). Terdapat korelasi positif dengan derajat lemah namun bermakna secara statistik antara nephrin serum dengan tekanan darah sistolik (r= 0,36; p= 0,02) namun tidak signifikan terhadap tekanan darah diastolik (r= 0,3; p= 0,05). Tidak ditemukan korelasi yang bermakna antara kadar nephrin serum dengan proteinuria (r= 0,18; p= 0,54).
Kesimpulan: Kadar nephrin serum pada kelompok preeklamsia berat lebih tinggi dibandingkan kehamilan normal dan terdapat korelasi antara nephrin serum dengan tekanan darah sistolik.

Kata kunci: nephrin serum, podosit, preeklamsia berat, proteinuria, tekanan darah

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Author Biographies

Sofie R. Krisnadi, Faculty of Medicine Universitas Padjadjaran Dr. Hasan Sadikin General Hospital Bandung

Department of Obstetrics and Gynecology

Hartanto Bayuaji, Faculty of Medicine Universitas Padjadjaran Dr. Hasan Sadikin General Hospital Bandung

Department of Obstetrics and Gynecology

Published
2021-01-15
Section
Research Article